A Process Development FOR Extraction of Marker Compound from EMBLICA OFFICINALIS FRUIT
نویسندگان
چکیده
The present study is done to examine and optimize the process of enzyme-assisted extraction of Gallic acid from the dried fruit of Emblica officinalis. The aqueous extraction yielded 12.09% of Gallic acid. A selective extraction process after the treatment with enzymes is proposed by using 30% (v/v) methanol which releases up to 15.68% of the Gallic acid, present in the dried fruit. The optimal conditions were as follows: pH value was 4.5, concentration of cellulase solution was 2.5 mg/mL, incubation time was 8 h, incubation temperature was 50 oC and solid:solvent ratio was 1:8 . Enzyme-assisted extraction was proven to be highly efficient in the process development for extraction of marker compounds from herbal drugs and could be used in making enriched herbal extracts of product on a large scale.
منابع مشابه
Dual-cholinesterase inhibition by an aqueous extract of Emblica officinalis fruit and its mode of inhibition by kinetic study
Emblica officinalis (Family: Euphorbiaceae) commonly known as Indian Gooseberry or Amla, is widely used in Ayurveda for its memory enhancing, anti-aging, anti-stress and antioxidant properties. This study evaluated the anti-cholinesterase activity of an aqueous extract from the fruits of Emblica officinalis as these are the key enzymes involved in cholinergic transmission. Cholinesterase inhibi...
متن کاملExtraction of Polyphenol, Flavonoid from Emblica officinalis, Citrus limon, Cucumis sativus and Evaluation of their Antioxidant Activity
The antioxidant potential of three commonly used edible fruits and vegetables Emblica officinalis (Amla), Citrus limon (lemon) and Cucumis sativus (cucumber) available in local market of Jaipur (India), was estimated. The parts of food material taken for this study is edible part of Emblica officinalis, peel of Citrus limon and Cucumis sativus. The total phenolic content, antoxidant activity an...
متن کاملComparative antimicrobial activities of Emblica officinalis and Ocimum sanctum
The aqueous and successive extracts of the fruit pulp of Emblica officinalis and fresh leaves and stems of Ocimum sanctum were prepared and evaluated for antimicrobial activity. The successive extracts such as petroleum ether,chloroform, ethyl acetate and methanol were prepared by successive solvent extraction method and aqueous extract by maceration process and screened for antimicrobial activ...
متن کاملRAPD Analysis for Determination of Components in Herbal Medicine
In this study, the RAPD (Random Amplified Polymorphic DNA) technique was employed for determination of the components in an Ayurvedic herbal prescription, Rasayana Churna. One-hundred-and-twenty decamer oligonucleotide primers were screened in the RAPD analysis to identify three Ayurvedic medicines, dried stem of Tinospora cordifolia, dried fruit of Emblica officinalis and dried fruit of Tribul...
متن کاملInduction of apoptosis of human primary osteoclasts treated with extracts from the medicinal plant Emblica officinalis
BACKGROUND Osteoclasts (OCs) are involved in rheumatoid arthritis and in several pathologies associated with bone loss. Recent results support the concept that some medicinal plants and derived natural products are of great interest for developing therapeutic strategies against bone disorders, including rheumatoid arthritis and osteoporosis. In this study we determined whether extracts of Embli...
متن کامل